Human chorionic gonadotropin from day 2 spent embryo culture media and its relationship to embryo development.

نویسندگان

  • Sivakumar Ramu
  • Brian Acacio
  • Mark Adamowicz
  • Sylvia Parrett
  • Rajasingam S Jeyendran
چکیده

OBJECTIVE To detect hCG in spent embryo culture media at day 2 after intracytoplasmic sperm injection and to assess the relationship of hCG to embryo development. DESIGN Experimental study. SETTING Fertility center and clinical diagnostic laboratory. SAMPLE(S) A total of 102 spent culture media from day 2 human embryos and corresponding unexposed media for blank control. INTERVENTION(S) The culture media samples were tested for hCG by ELISA. MAIN OUTCOME MEASURE(S) Quantity of hCG produced by embryos and correlation with the embryos' developmental status. RESULT(S) hCG was found in 93 of 102 culture media tested by enzyme-linked immunosorbent assay. The correlation analysis revealed that the concentration of hCG was independent of embryo developmental status. CONCLUSION(S) The ability to detect hCG from day 2 spent culture media may be used as a marker for embryo competence.

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عنوان ژورنال:
  • Fertility and sterility

دوره 96 3  شماره 

صفحات  -

تاریخ انتشار 2011